SDS-PAGE OF PROTEIN IN MYELIN:
The
total protein present in stress induced and normal rats were studied by
subjecting it in to polyacrylamide gel electrophoresis followed by CBB staining.
The band pattern shown in plate 1 shows appreciable changes in the fore, mid,
hind brain region of stress induced rats when compared to the control group of
rats. High density bands were encountered in FB, MB and HB while the stress
induction resulted in low intense bands.
SDS-PAGE PATTERN OF
MYELIN PROTEIN
|
ESTIMATION OF ATPase FROM MYELIN:
Table
3 depict the activity of ATPases (Na-K+.Mg2+, Ca2+)
in rat brain myelin were measured. The activity of ATPase were found to be
decreased in the stress group rats than the control rats (p<0.05).In
contrast, Group II showed significant increased activities of all (p<0.05).
ATPases.
ATPases are highly sensitive to ischemic
stress. The most prominent disturbences in energy metabolism are exposed as
depletion of adenosine triphosphate (ATP); the consequence in inhibition of all
ATP-dependent enzymes (Stojanovic; 1988). Dopamine and Serotonin known to
excite/inhibit synaptosomal Na-K+TPase in the absence /presence of
Ca2+ (Glebov et al., 1997).
Na-K+TPase
is crucial for maintaining ionic gradients in neurons and is reported to be
eritically involved in potassium buffering after periods of hyper stimulation
when our physiology esxcited to hyper state.
The
pathophysiology of some psychiatric disorders is believed to be associated with
perturbation of ion homostasis, and earlier studies have shown that activity is
decreased in patients with depression and other psychiatric disorders (Wood et al., 1991).
TABLE 3
The
activities of ATPases of myelin of fore, mid and hind brain of control and
experimental Rats.
PARTICULARS
|
GROUR
I
|
GROUP II
|
||||
FB
|
MB
|
HB
|
FB
|
MB
|
HB
|
|
Na-k 2+
|
0.07 ± 0.03
|
0.06± 0.02
|
0.05 ± 0.02
|
0.07 ± 0.03
|
0.04 ± 0.02
|
0.03 ± 0.02
|
Mg2+
|
0.06 ±0.04
|
0.05 ± 0.03
|
0.03 ± 0.05
|
0.05 ± 0.02
|
0.04 ± 0.05
|
0.02 ± 0.04
|
Ca2+
|
0.08 ± 0.03
|
0.07 ±0.02
|
0.05 ± 0.04
|
0.07 ± 0.02
|
0.06 ± 0.05
|
0.04 ± 0.04
|
Values are expressed as mean ± SD (n=6)
ATPase - µmoles of phosphorus liberated/mg protein
/min.
Statistical representation was expressed as P<0.05
*a Group I compared with Group II
Graph
-4
The
activities of Na+-K2+ ATPases of myelin of fore, mid and
hind brain of control and experimental Rats.
Values are expressed as mean ± SD (n=6)
ATPase - µmoles of phosphorus liberated/mg protein /min.
Statistical representation was expressed as P<0.05
*a Group I compared with Group II
Graph -5
The
activities of Ca2+ ATPases of myelin of fore, mid and hind brain of
control and experimental Rats.
Values are expressed as mean ± SD (n=6)
ATPase - µmoles of phosphorus liberated/mg protein
/min.
Statistical representation was expressed as P<0.05
*a Group I compared with Group II
The
activities of Mg2+ ATPases of myelin of fore, mid and hind brain of
control and experimental Rats.
Values are expressed as mean ± SD (n=6)
ATPase - µmoles of phosphorus liberated/mg protein
/min.
Statistical representation was expressed as P<0.05
*a Group I compared with Group II
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