Glyprotein components Of Myelin Protein in Experimental Models of Stress SCOPE OF THE STUDY




SCOPE OF THE STUDY:                  
 Stress in the present times is an uprising area of interest as its effect is seen in all age groups. It has been observed to affect many memory functions and cognitive functioning of the brain. There are different levels of stress and the high levels can be intrinsic or extrinsic. Intrinsic stress level is triggered by a cognitive challenge whereas extrinsic can be triggered by a condition not related to a cognitive task. Intrinsic stress can be acutely and chronically experienced by a person. The varying effects of stress on performance or stress hormones are often compared to or known as “inverted-u” which induces areas in learning, memory and plasticity.  Chronic stress can impact the brain structure and cognitions of the brain.
Stress proteins are constitutively expressed in normal CNS tissues, in a variety of cell types (oligodendrocytes, astrocytes and neurons). Their function is uncertain but they may be critical during nervous system development and may protect cells from various stresses.
The compact myelin sheath formed around nerve axons speeds up nerve conduction and also nurtures the axon. Destruction of this sheath in demyelinating diseases results in nerve conduction failure and neurodegeneration. Myelin basic protein (MBP) is the second most abundant protein of central nervous system (CNS) myelin (after the proteolipid protein), representing about 30 % of the total myelin protein and about 10 % of myelin by weight. It is also present in peripheral nervous system (PNS) myelin but as a lower percentage of the total protein.
In nerve tissue glycoproteins are abundant in gray matter and appear to be associated with synaptosomes, axons, and microsomes. The large number of glycoproteins associated with central-nervous-system myelin suggests an unsuspected complexity in the interactions involved in the development and maintenance of plasma membrane.




In the present study, an attempt has been made to investigate the changes in glycoprotein components of myelin in fore brain, mid brain, and hind brain in light induced stress using animal model.

Outline plan of investigation,
§  Assay the behavioral changes.
§  Histopathological analysis.
§  Biochemical analysis (ATPase enzyme assays).
§  SDS - PAGE pattern of protein.
§  Estimation of Protein-bound carbohydrates
§  PAS pattern of Glycoprotein.


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