SCOPE OF
THE STUDY:
Stress in the present times is an uprising area of interest as its
effect is seen in all age groups. It has been observed to affect many memory
functions and cognitive functioning of the brain. There are different levels of stress and
the high levels can be intrinsic or extrinsic. Intrinsic stress level is
triggered by a cognitive challenge whereas extrinsic can be triggered by a
condition not related to a cognitive task. Intrinsic
stress can be acutely and chronically experienced by a person. The varying
effects of stress on performance or stress hormones are often compared to or
known as “inverted-u” which induces
areas in learning, memory and plasticity.
Chronic stress can impact the brain structure and cognitions of the brain.
Stress proteins are constitutively expressed in
normal CNS tissues, in a variety of cell types (oligodendrocytes, astrocytes
and neurons). Their function is uncertain but they may be critical during
nervous system development and may protect cells from various stresses.
The compact myelin sheath formed around nerve
axons speeds up nerve conduction and also nurtures the axon. Destruction of
this sheath in demyelinating diseases results in nerve conduction failure and
neurodegeneration. Myelin basic protein (MBP) is the second most abundant
protein of central nervous system (CNS) myelin (after the proteolipid protein),
representing about 30 % of the total myelin protein and about 10 % of myelin by
weight. It is also present in peripheral nervous system (PNS) myelin but as a
lower percentage of the total protein.
In nerve tissue
glycoproteins are abundant in gray matter and appear to be associated with
synaptosomes, axons, and microsomes. The large number of glycoproteins
associated with central-nervous-system myelin suggests an unsuspected
complexity in the interactions involved in the development and maintenance of
plasma membrane.
In the present study, an attempt has been made to
investigate the changes in glycoprotein components of myelin in fore brain, mid
brain, and hind brain in light induced stress using animal model.
Outline plan of investigation,
§ Assay the behavioral changes.
§ Histopathological analysis.
§ Biochemical analysis (ATPase enzyme assays).
§ SDS - PAGE pattern of protein.
§ Estimation of Protein-bound carbohydrates
§ PAS pattern of Glycoprotein.
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