RESULT AND DISCUSSION-BIOCHEMICAL PROFILE:

 


BIOCHEMICAL PROFILE:
Total protein levels were estimated in the myelin membrane of the brain cells obtained from all groups of rats. In Table.2, the results of the assay in group II rats under stress showed increased levels of protein in myelin conforming that considerable membrane damage has occurred due to lipid per oxidation in these groups reflecting in the protein levels when compared to control. As promotion of inflammatory destruction of protein caused by depression may get to negatively influence cardiovascular health (Jesse Stewart 2009).


ENZYME ASSAY:
TABLE-2
The total protein in the myelin 0f control and experimental rats
PARTICULARS
GROUP I
GROUP II
FB
MB
HB
FB
MB
HB

Myelin

61.36 ± 2.2

62.31 ± 2.0

62.40 ± 2.1

52.53 ± 1.8**a

45.71 ± 2.5***b

55.41 ± 2.2*c
         
PROTEIN

851.26 ±20.0

862.32 ± 14.0

866.55 ± 14.3

 801.26 ± 25.5**a

643.41 ± 15.5***b

800.34 ±
1 5.6*c

Values are expressed as mean ± SD (n=6)
Myelin – mg / 250mg
Protein – µg / mg of myelin
Statistical representation was expressed as p<0.05
*a FB of group I compared with Group II
*b MB of group I compared with Group II
*c HB of group I compared with Group II
 
Graph 2
Myelin weight of fore, mid and hind brain of control and experimental Rat.
Values are expressed as mean ± SD (n=6)
Myelin – mg / 250mg
Statistical representation was expressed as p<0.05
*a FB of group I compared with Group II
*b MB of group I compared with Group II
*c HB of group I compared with Group II

Graph 3
The total protein in myelin of fore, mid and hind brain of control and experimental Rat.
Values are expressed as mean ± SD (n=6)
Protein – µg / mg of myelin
Statistical representation was expressed as p<0.05
*a FB of group I compared with Group II
*b MB of group I compared with Group II
*c HB of group I compared with Group II


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